Journal of Marine Science and Technology

Journal of Marine Science and Technology

Comparative Antioxidant Activities and Polyphenolic Content of Marine actinomycetes from Deylam nearshore sediments

Document Type : Original Manuscript

Authors
Masoomeh Nateghzadeh 1
Soheila Matroodi 1
Kamahldin Haghbeen 2
1 Department of Marine biology, Faculty of Marine Science and oceanography, Khorramshahr University of marine science and technology, Iran.
2 National Institute of Genetic Engineering and Biotechnology
10.22113/jmst.2018.104541.2073
Abstract
Antioxidant used for chronic disease as treatment and for repalcing artificial antioxidant with natural antioxidant; To investigate antioxidant activity, we used marine actinomycetes as an important resources of bioactive compounds.. In this study, for phylogenetic analysis of 15 marine actinomycetes isolated from Deylam intertidal sediments, 16S rDNA sequences were used. In addition, the total phenolic content (TPC) was determined with the Folin-Ciocalteu’s reagent using gallic acid as a standard and the results were expressed as mM of gallic acid. Then, the antioxidant potential of the extracts was determined by analytical methods, ferric reducing antioxidants power (FRAP). The antioxidant potential of the crude extracts exhibited strong ferric reducing power activity at 517nm and the reducing power activity was strongly correlated with the total phenolics content of the isolates. Among the tested isolates, AHA1 (1419 µM) and AMJ1 (608.8 ) showed the hieghest and lowest total phenolic content, respectivly. Moreover AHA3 and AMJ5 (230.66 and 75.57µM) showed the highest and lowest reducing power, respectivly. These strains showed acceptable antioxidant activity and could be good candidates for more studies as nature resources of pharmaceutical antioxidant compound.
Keywords
Marine actinomycetes
Antioxidant activity
FRAP
Total phenolic Content (TPC)

Subjects

Alijani H, Matroodi S, sharafi A, zamani I. 2017. Diversity and Antimicrobial Activities of Streptomyces Isolated from intertidal Sediments of Deylam, Iran. Razi J Med Sci. 24 (162): 22-31.
Arabsorkhi F., Safaeian  S.,  Salimi  L.2015. The Comparison of Antioxidant Property of Enriched Jelly Gum with Chlorella vulgaris and Normal Jelly Gum. J marine sci technol. 12(1).
Baltz RH. 2008. Renaissance in antibacterial discovery from actinomycetes. Curr Opin Pharmacol. 8:1-7.
Benzie FF., Szeto YT. 1999. Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. J Agri Food Chem. 47(2): 633–636.
Benzie IF., Strain JJ., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analy biochem. 239(1):70-76.
BerdyJ. 2005. Bioactive Microbial Metabolites. J Antibio. (Tokyo). 58:1-26.
Bull A.T., Stach JEM. 2007. Marine actinobacteria: new opportunities for natural product search and discovery. Trend Microbiol.15:491-9.
Dekkers J., van Doornen L., Kemper H. 1996. The role of antioxidant vitamins and enzymes in the prevention of exercise–induced muscle damage. Sports Med. 21: 213–238.
Gulluce M., Sahin F., Sokmen M., Ozer H., Daferera D., Sokmen A., Polissiou M., Adiguzel A. Ozkan H. 2007. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food chem. 103(4): 1449-1456.
Heidari M., Zolgharnin H., Sakhaei N., Mirzaei A., Movahedinia A. 2015. Antioxidant capacity and phenolic and flavonoid content of macro algae in the northern coasts of the Persian Gulf in Bushehr province. J marine sci technol.  14(1).
 
Irshad M., Singh M., Zafaryab M., Rizvi MMA. 2012. Comparative analysis of the antioxidant activity of Cassia fistula extracts. Inter J Med Chem. doi:10.1155/2012/157125.
Katalinic V., Milos M., Kulisic T., Jukic M. 2006. Screening of 70 medicinalplant extracts for antioxidant capacity and total phenols. Food Chem. 94:550-7.
Jabari M, Matroodi S, Zolgharnein H, Sharafi A, Zamani I. 2016. Screening, isolation and study of antifungal activity of marine actinomycetes from Deylam nearshore sediments. Iran J Med Microbiol. 9 (4) :87-94.
Kekuda PTR, Rakesh KN., Junaid S. Kekuda PT. 2013. Antibacterial and antioxidant activities of Streptomyces species SRDP-H03 isolated from soil of Hosudi, Karnataka, India. J Drug Deliv Sci Technol. 3(4): 47-53.
Lakhtakia R., Ramji MT., Lavanya K., Rajesh K., Jayakumar K., Sneha C., Narayan A., Ramya B., Ramana G., Chari PVB., Chaitanya KV. 2011. The role of antioxidants in human health maintenance: Small molecules with infinite functions. IJPSR.  2:1395-402.
Markovic ZS., Manojlovic NT. 2010. Analytical characterizationof lichexanthone in lichen: HPLC, UVspectroscopic and DFT analysis of lichexanthone extracted from Laurera benguelenisis(Mull. Arg.). Zahlbr Monatshefte Chemie. 141:945-52.
Mladenka P., Zatloukalova L., Filipsky T., Hrdina R. 2010. Cardiovascular effects of flavonoids are not caused only by direct antioxidant activity. Free Radical Bio Med. 49(6):963–75.
Nagaseshu P., Gayatridevi V., Anil Kumar B., Seema Kumari, Murali Mohan G., Rama Rao Malla. 2016. Antioxidant And Antiproliferative Potentials Of Marine Actinomycetes. Inter J Pharma Pharmaceut Sci. 8(8): 277-284.
Naine SJ., Devi CS., Mohanasrinivasan V. 2015. Antimicrobial, Antioxidant and Cytotoxic Activity of Marine Streptomyces parvulus VITJS11 Crude Extract. Brazilian Arch Biol Technol. 58(2): 198-207.
Nemzer BV., Rodriguez LC., Hammond L., DiSilvestro R., Hunter JM., Pietrzkowski Z. 2011. Acute reduction of serum 8-iso-PGF2-alpha and advanced oxidation protein products in vivo by a polyphenol-rich beverage; a pilot clinical study with phytochemical and in vitro antioxidant characterization. Nutr J 10(1):1–11.
Nonomura H., Ohara Y. 1974. Distribution of actinomycetes in soil. XII. A new species of actinomycetes, Thermononospora mesouviformis, sp. nov. J Ferment Technol. 52: 10–13.
Pham-Huy LA., He H., Pham-Huyc C. 2008. Free radicals, antioxidants in disease and health. Int J Biomed Sci. 4:89-96.
Ramesh S. 2009. Marine actinomycetes diversity in Bay of Bengal, India: isolation and characterization of bioactive compounds from Streptomyces fungicidicus MML 1614. India. Dissertation, University of Madras, Chennai, India.
Refrenece
Rice-Evans CA., Miller NJ., Paganga G. 1997. Antioxidant properties of phenolic compounds. Trends Plant Sci. 4: 304–309.
Ross JA., Kasum CM. 2002. Dietary flavonoids: Bioavailability, metabolic effects, and safety. Annu Rev Nutr. 22:19–34.
Sandoval-Acuna C., Ferreira J., Speisky H. 2014. Polyphenols and mitochondria: an update on their increasingly emerging ROS-scavenging independent actions. Arch Biochem Biophys. 559:75–90.
Sanjivkumar M., Babu DR., Suganya AM., Silambarasan T., Balagurunathan R., Immanuel G., 2016. Investigation on pharmacological activities of secondary metabolite extracted from a mangrove associated actinobacterium Streptomyces olivaceus (MSU3). Biocata Agri Biotechnol. 6: 82-90.
Singh SB., Pelaez F. 2008. Biodiversity, chemical and drug discovery. Progr Drug Res. 65:143-74.
Singleton VL., Orthofer R., Lamuela-Raventós RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Meth enzymol.  299: 152-178.
Tamura K., Dudley J., Nei M. Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecul Boil Evol.24(8): 1596-1599.
Th´eriault M., Gaillett S., Kermasha S., Lacroix M. 2006. Antioxidant, antiradical and antimutagenic activities of phenolic compounds present in maple products. Food Chem. 98: 490–501.
Thenmozhi M., Kannabiran K. 2012. Antimicrobial and antioxidant properties of marine actinomycete Streptomyces sp. VITSTK7. Oxidant. Antioxidant Med Sci. 1: 51-57.
Thenmozhi M., Sindhura S., Kannabiran K. 2010. Characterization of Antioxidant activity of Streptomyces species VITTK3 isolated from Puducherry Coast, India. J Adv Sci Res. 1:46-52.
Zhang Z., Liao L., Moore J., Wu T., Wang Z. 2009. Antioxidant phenolic compounds from walnut kernels (Juglans regia L.). Food Chem. 113(1): 160-1

  • Receive Date 05 November 2017
  • Revise Date 30 September 2018
  • Accept Date 02 October 2018
  • Publish Date 23 August 2019