Journal of Marine Science and Technology

Journal of Marine Science and Technology

Antioxidant and antibacterial assay of two red marine macro-alga of Bandar Abbas coastal

Document Type : Original Manuscript

Authors
1 Young Researchers and Elite Club, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran.
2 Department of Marine Biology, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran.
3 Ariculture and Natural Resources Researches Center of Hormozgan, Bandar Abbas, Iran.
4 Department of Polymer Engineering, Graduate University of Advanced Technology, Kerman, Iran.
Abstract
Seaweed is favored seafood in some regions and is also used as feedstock for extracting fine chemicals. The total global seaweed production continues to grow This study investigated the biological activities of n-Hexane, Ethyl‌acetate and Methanol extract of two red marine macro algae (Gracilariopsis persica and Hypnea flagelliformis), collected from the coast of Bandar Abbas, Persian Gulf, Iran. For identification the superior species with biological properties, the tested activities included Carotenoids content, total Phenolic content, total flavonoids content, antioxidant activity at the concentration (3 mg/ml) by ferric reducing power (FRP) and total antioxidant capacity (TAC) assay and at final, antibacterial activity was evaluated. This study revealed that the more effective macro algae extracts by maximum antioxidant capacity: FRP and TAC, were recorded for Ethyl‌acetate extracts. The result showed the highest content of phenolic and flavonoid compounds were recorded for the Methanol extracts of Gp. persica, 45.12±0.01 (mg GA/gr DW) and 2.28±0.007 (mg QE /gr DW), respectively while H. flagelliformis showed the maximum Carotenoid content 17±0.06 (mg 100g-1). In addition, the highest antibacterial activity was recorded for the n-hexane and followed by Ethyl‌acetate extracts. In general comparison, though, according to the results, antioxidant and antibacterial activity of species in this study were calculated less than standard, but could be accounted these seaweeds as safe biological properties and with abundance of them in coastal of Bandar Abbas, could be considered for future applications in medicine and dietary supplements.
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Al-Saif SS., Abdel-Raouf N., El-Wazanani HA. and Aref IA. 2014. Antibacterial substances from marine algae isolated from Jeddah coast of Red sea, Saudi Arabia. Saudi J Biol Sci. 21(1): 57-64.
Amsler CD. and Fairhead VA. 2005. Defensive and sensory chemical ecology of brown algae. Adv Bot Res. 43: 1-91.
Arnon DI. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24(1): 1.
Athukorala Y., Lee KW., Kim SK. and Jeon YJ. 2007. Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea. Biores Technol. 98(9): 1711-1716.
Bahorun T., Gressier B., Trotin F., Brunet C., Dine T., Luyckx M., Vasseur J., Cazin M., Cazin J. and Pinkas M. 1996. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneim Forsch. 46(11): 1086-1089.
Bauer A., Kirby W., Sherris JC. and Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am J Clinic Pathol.45(4):493.
Bellorin AM., Buriyo A., Sohrabipour J., Oliveira MC. and Oliveira EC. 2008. Gracilariopsis Mclachlaniisp. Nov. Andgracilariopsis persicasp. Nov. Of the Gracilariaceae (Gracilariales, Rhodophyceae) from the Indian Ocean. J Phycol. 44(4): 1022-1032.
Benkendorff K., Davis AR., Rogers CN. and Bremne, JB. 2005. Free fatty acids and sterols in the benthic spawn of aquatic molluscs, and their associated antimicrobial properties. J Exp Mar Biol Ecol. 316(1): 29-44.
Chandini SK., Ganesan P. and Bhaskar N. 2008. In vitro antioxidant activities of three selected brown seaweeds of India. Food Chem. 107(2): 707-713.
Chinnadurai S. and Kalyanasundaram G. 2013. Estimation of major pigment content in seaweeds collected from Pondicherry coast. Int J Sci Tech. 9(1): 522-525.
Connan S., Deslandes E. and Gall EA. 2007. Influence of day–night and tidal cycles on phenol content and antioxidant capacity in three temperate intertidal brown seaweeds. JExp Mar Biol Ecol. 349(2): 359-369.
El Gamal AA. 2010. Biological importance of marine algae. Saudi Pharm J. 18(1): 1-25.
Ganesan P., Kumar CS. and Bhaskar N. 2008. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Biores Technol. 99(8): 2717-2723.
Gohari AR., Hadjiakhoondi A., Sadat-Ebrahimi E., Saeidnia S. and Shafiee A. 2005. Cytotoxic terpenoids from Satureja macrantha CA Mey. DARU J Pharm Sci. 13(4): 177-181.
Hanson JR. 2003. Natural products: the secondary metabolites. Royal Society of Chemistry. Vol. 17.
Heidari M., Zolgharnine H., Sakhaei N., Mirzaei A., movahedinia A.  2015. Antioxidant capacity and phenolic and flavonoid content of macro algae in the
northern coasts of the Persian Gulf in Bushehr province.  J Mar Sci Tech. 14(2):54-45.
Jeliani ZZ., Yousefzadi M., Sohrabipour J. and Toiserkani H. 2017. Growth, phytochemicals, and optimal timing of planting Gracilariopsis persica: an economic red seaweed. J Appl Phycol. 1-9.
Kirk J. and Allen R. 1965. Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochem Biophys Res Commun. 21(6): 523-530.
Kohen R. and Nyska A. 2002. Invited review: Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol pathol. 30(6): 620-650.
Kumar KS., Ganesan K. and Rao PVS. 2008. Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty–An edible seaweed. Food Chem. 107(1): 289-295.
Matsukawa R., Dubinsky Z., Kishimoto E., Masaki K., Masuda Y., Takeuchi T., Chihara M., Yamamoto Y., Niki E. and Karube I. 1997. A comparison of screening methods for antioxidant activity in seaweeds. J Appl Phycol. 9(1): 29-35.
Mitsuda H. 1966. Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo shokuryo. 19: 210-221.
Mochtar AH., Parawansa I., Ali MSS., Jusoff K., Reta R., Astuti SD., Azis N. and Muchdar A. 2013. Effects of Harvest Age of Seaweed on Carragenan Yield and Gel Strength. World Appl Sci J. 26:13-16.
National Committee for Clinical Laboratory Standards (NCCLS). 1997. Performance standards forantimicrobial disk susceptibility test. 6th edition. Approved Standard. M 100-A6. Wayne, Pensylvania, USA.
Oh JK., Drumright R., Siegwart DJ. and Matyjaszewski K. 2008. The development of microgels/nanogels for drug delivery applications. Prog Polym Sci.33(4): 448-477.
Oranday M., Verde M., Martinez-Lozano S. and Waksman N. 2004. Active fractions from four species of marine algae. Phyton (Buenos Aires). 73: 165-170.
Peschel W., Sánchez-Rabaneda F., Diekmann W., Plescher A., Gartzía I., Jiménez D., Lamuela-Raventos R., Buxaderas S. and Codina C. 2006. An industrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chem. 97(1): 137-150.
Plaza M., Cifuentes A. and Ibáñez E. 2008. In the search of new functional food ingredients from algae. Trends Food Sci Technol. 19(1): 31-39.
Safari P., Rezaei M., Shaviklo AR., garmsiri A., Babakhani A. 2015. In vitro antioxidative activity and total phenolic content etermination of two Persian Gulf seaweed species Chaetomorpha sp and Colpomenia sinuosa. J Mar Sci Tech. 14(1):64-77.
Seenivasan R., Indu H., Archana G. and Geetha S. 2010. The antibacterial activity of some marine algae from south east coast of India. J Phar Res. 8: 1907-1912.
Singleton V. and Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult. 16(3): 144-158.
Sohrabipour J., Nejadsatari T., Assadi M. and Rabei R. 2004. The marine algae of the southern coast of Iran, Persian Gulf, Lengeh area. Iran Journ Bot. 10(2): 83-93.
Sohrabipour J. and Rabei R. 2008. Rhodophyta of Oman Gulf (South East of Iran). Iran Journ Bot. 14(1): 70-74.
Toyosaki T. and Iwabuchi M. 2009. New antioxidant protein in seaweed (Porphyra yezoensis Ueda). Int J Food Sci Nutr. 60(sup2): 46-56.
Valente LMP., Rema P., Ferraro V., Pintado M., Sousa-Pinto I., Cunha LM., Oliveira MB. and Araújo M. 2015. Iodine enrichment of rainbow trout flesh by dietary supplementation with the red seaweed Gracilaria vermiculophylla. Aquaculture. 446: 132-139.
Volk RB. 2006. Antialgal activity of several cyanobacterial exometabolites. J Appl phycol. 18(2): 145-151.
Yoshida M. 1959. Naphthaquinone pigments in Psammechinus miliaris (Gmelin). J Mar Biol Assoc U. K. 38(03): 455-460.
Yousefzadi M., Riahi-Madvar A., Hadian J., Rezaee F., Rafiee R. and Biniaz M. 2014. Toxicity of essential oil of Satureja khuzistanica: In vitro cytotoxicity and anti-microbial activity. J Immunotoxicol. 11(1): 50-5.
Zubia M., Robledo D. and Freile-Pelegrin Y. 2007. Antioxidant activities in tropical marine macroalgae from the Yucatan Peninsula, Mexico. J Appl Phycol. 19(5): 449-458.

  • Receive Date 15 June 2016
  • Revise Date 06 March 2017
  • Accept Date 06 March 2017
  • Publish Date 23 July 2018