Journal of Marine Science and Technology

Journal of Marine Science and Technology

Introducting the best cell culture method for primary hepatocyte from orange-spotted Grouper, Epinephelus coioides

Document Type : Original Manuscript

Authors
1 Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technolgy
2 1. Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technolgy 2.Department of Marine Biology, Faculty of Marine Sciences, University of Mazandaran, Babolsar
3 1. گروه زیست شناسی دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر
4 Jondi Shapour University of Medical Sciences, Ahvaz
Abstract
Liver is one of the most important organs in vertebrates that have important roles in detoxifying. The main purpose of the present study was developing appropriate methodology for the primary cultivation of hepatic cells from orange-spotted Grouper, Epinephelus coioides. In present study, hepatocytes were isolated from five grouper individuals. Initially, the fish wiped with 70% ethanol. Liver were removed and cut into small pieces with scissors and hepatocytes were disconnected using different enzymatic digestion with collagenase (Type 1 and 4) and trypsin and additional nutrient materials in culturing mediums. Then, cells were cultured for 2 weeks in Lebowitz L-15 under 3 methods: 1. Using enzymatic digestion by trypsin, 2. Using enzymatic digestion by collagenase (type 1 and 4) and 3. Using nutrients and additives was cultured. Finally, effects of different incubation temperature (20, 25, 28, 30 and 32 degree of Celsius) and Bovine serum content (0, 10 and 20% and 20%+ITS) on cell growth were estimated. According to the results, digestion by collagenase type 4, resulted in more cell colonization and growth in comparison with other methods.In conclusion, the best culture method for primary hepatocyte from orange-spotted Grouper, Epinephelus coioides., was using ITS+20%FBS under 30 degree of Celsius incubation temprature.
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Volume 16, Issue 1 - Serial Number 1
Summer 2017
Pages 102-111

  • Receive Date 16 September 2015
  • Revise Date 04 October 2015
  • Accept Date 25 January 2016
  • Publish Date 21 April 2017