Journal of Marine Science and Technology

Journal of Marine Science and Technology

The effect of temperature changes to reduce toxic jellyfish Crambionella orsini

Document Type : Original Manuscript

Authors
niloofar saki 1
yadollah nikpour ghanavaty 2
ahmad taghavi moghadam 3
kamal ghanemi 4
1 Department of Marine Chemistry, Faculty of Marine Science and Technology, KHORAMSHAHR
2 khoramshahr university of marine and science technology
3 razi vaccine and serum research institute
4 khoramshahr university of marine science and technology
10.22113/jmst.2016.41173
Abstract
The aim of this study is evaluate the effect of temperature changes to reduce toxicity, jellyfish venom Crambionella orsini. Venom extraction was done according to Bloom method. To breaking wall of nematocysts capsule, at first, sonication it and than The resulting solution was centrifuged. To evaluate the effect of temperature on the venom, it is heated at different temperatures and then injected into SoRI mice. After catching jellyfish Crambionella orsini from Arvand nidstream estuary umbrellas edge and tentacles jellyfish were separated and put were in water that was removed on the same area. Toxins LD50 were calculated by Jung and Choi method .and using statistical analysis to obtain minimal lethal dose of poison done by the 2007Excel. The results showed that the venome of jellyfish Crambionella orsini, like venome of other animals is, based on a protein and that is sensitive to heat. This venome at a temperature of 48 °C is disabled and lose their structure and also its minimum lethal dose is 0.5 ml.
Keywords
jellyfish Crambionella orsini
Arvand
extracting venom
cnidarian
C.fleckeri

Subjects

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Journal of Marine Science and Technology
Volume 15, Issue 2
Autumn 2016
Pages 143-166

  • Receive Date 06 December 2015
  • Revise Date 18 January 2016
  • Accept Date 25 December 2016
  • Publish Date 22 August 2016