Journal of Marine Science and Technology

Journal of Marine Science and Technology

Assay of Genetic diversity of cuttlefish (Sepia pharaonis ;Ehrenberg, 1831) populations using microsatellite markers in Bandarabass and Bushehr regions

Document Type : Original Manuscript

Authors
hedayat yavarmoghadam 1
h zolgharnein 2
Mohammad Ali Salari Aliabadi 3
s keyvan 4
Mohamad Modarresi 5
1 College of Marine Science, Department of Marine Biology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khouzestan, Iran
2 گروه زیست شناسی دریا، دانشکده علوم دریایی و اقیانوسی، دانشگاه علوم و فنون دریایی خرمشهر، خرمشهر
3 Department of Marine Biology, College of Marine Science, Khorramshahr Marine Science and Technology University, P.O. Box: 669, Khorramshahr, Khuzestan, Iran.
4 College of Marine Science, Department of Fisheries, Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khouzestan, Iran
5 Persian Gulf Research and Studies Center, Persian Gulf University, Boushehr, Iran
10.22113/jmst.2016.38445
Abstract
In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology laboratory of Khorramshahr University of Marine Science and Technology. Genomic DNA was extracted with CTAB method. The quality and quantity of extracted DNA was assessed by 1% agarose gel electrophoresis and spectrophotometry, respectively. Polymerase chain reaction conducted with 6 pairs of microsatellite primers. PCR products were electrophoresised on 8% polyacrylamide gel and stained with silver nitrate. These primers were shown 4 pairs of polymorph and 2 pairs of monomorp. Allele Sizes were measured in populations then genetic parameter were calculated using Arlequin and Gen Alex Programs and phylogenetic relationship was determinated and drawn using TFPGA Program. Result obtained showed genetic distance and resemblance distance is 0.282 and 0.754, respectively and genetic differentiation was present 0.031. The findings of present study showed low levels of genetic differentiation but significant between of populations and S. pharaonis were likely two different population. These findings provide useful information for conservation and management of this species in the Persian Gulf.
Keywords
Microsatellite
genetic diversity
Sepia pharaonis
Bandarabass
Bushehr

Subjects

Adcock, G.J., Shaw, P.W., Rodhouse, P.G., Carvalho, G.R. 1999. Microsatellite analysis of genetic diversity in the squid Illexargentinus during a period of intensive fishing.Marine Ecology Progress Series. 187: 171-178.
Anderson, F.E., Valinassab, T., Ho, C.W., Mohamed, K.S., Asokan, P.K., Rao, G.S., Nootmorn, P., Chotiyaputta, C., Dunning, M., Lu, C.C. 2007.Phylogeography of the pharaoh cuttle Sepia pharaonis based on partial mitochondrial 16S sequence data. Reviews in Fish Biology and Fisheries. 17: 345-352.
Bassam, B. J., Caetano-Anolles, G. and Gresshoff, G. M. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical biochemistry 84: 680-683.
Belkhir, K., Borsa, P., Chikhi, L., Raufaste, N., Bonhomme, F. 2004. GENETIX 4.05, Population genetics software for Windows TM. Université de Montpellier II. Montpellier.
Boal, J.G., Prosser, K.N., Holm, J.B., Simmons, T.L., Haas, R.E., Nagle, G.T. 2010. Sexually mature cuttlefish are attracted to the eggs of conspecifics. Journal of chemical ecology. 36: 834-836.
 Boyle, P., Boletzky, S. 1996. Cephalopod populations: definition and dynamics. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences. 351: 985-1002.
 Carpenter, K., Krupp, F., Jones, D., Zajonz, U. 1997.FAO species identification field guide for fishery purposes.The living marine resources of Kuwait, Eastern Saudi Arabia, Bahrain, Qatar, and the United Arab Emirates.
Diz, A.P., Presa, P. 2009. The genetic diversity pattern of Mytilus galloprovincialis in Galician Rías (NW Iberian estuaries). Aquaculture. 287: 278-285.
Doubleday, Z.A., Semmens, J.M., Smolenski, A.J., Shaw, P.W. 2009. Microsatellite DNA markers and morphometrics reveal a complex population structure in a merobenthic octopus species (Octopus maorum) in south-east Australia and New Zealand. Marine biology. 156: 1183-1192.
Garoia, F., Guarniero, I., Ramšak, A., Ungaro, N., Landi, M., Piccinetti, C., Mannini, P., Tinti, F. 2004. Microsatellite DNA variation reveals high gene flow and panmictic populations in the Adriatic shared stocks of the European squid and cuttlefish (Cephalopoda). Heredity. 93: 166-174.
Hickman, C. P., Roberts, L. S and Larson, A. 2003. Animal Biodiversity. 464p
Khodadadi R., Yahyavi, M., Ghorbani, R., Shabani, M.J .2010. Spawning season and fecundity of Sepia pharaonis In Bushehr coastal waters (Persian Gulf). Iranian Scientific Fisheries Journal.19:31-38.
Lindgren, A.R., Giribet, G., Nishiguchi, M. 2004.A combined approach to the phylogeny of Cephalopoda (Mollusca).Cladistics. 20: 454-486.
Miller, M.P. 1997. Tools for population genetic analyses (TFPGA) 1.3: A Windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author.4, 157.
Nahavandi, R .Rezvani, S. Vousoghi, Gh. Kazemi, B. 2005. Gene 18S rRNA variation  of cuttle fish of population (Sepia pharaonis) in Persian Gulf and Oman sea using PCR-RFLP method. Iranian Scientific Fisheries Journal.14:157-168.
Nei, M. 1972. Genetic distance between populations.American naturalist. 283-292.
Oosterhout, C. V., Hutchinson, W. F., Wills, D.  P. M. and Shipley, P. 2004. Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes. 4: 535-538.
Peakall, R., Smouse, P.E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research.Molecular Ecology Notes. 6: 288-295.
Shaw, P. 2003. Polymorphic microsatellite DNA markers for the assessment of genetic diversity and paternity testing in the giant cuttlefish, Sepia apama (Cephalopoda). Conservation Genetics. 4: 533-535.
Shaw, P., Pérez Losada, M. 2000.Polymorphic microsatellites in the common cuttlefish Sepia officinalis (Cephalopoda).Molecular Ecology. 9: 237-238.
Wang, H., Lin, L., Liu, S.F., Jiang, Z.Q. 2010. Isolation and characterization of polymorphic microsatellite loci in golden cuttlefish (Sepia esculenta). Molecular Ecology Resources, 1-9.
Weir, B.S., Cockerham C.C. 1984. Estimating F-statistics for the analysis of population structure. Evolution, 38:1358–1370.
Winnepenninckx, B., Backeljau, T., Dewachter, R. 1993. Extraction of high-molecular-weight DNA from mollusks.TRENDS in Genetics.9, 407.
Wolfram, K., Mark, F.C., John, U., Lucassen, M., Pörtner, H.O. 2006. Microsatellite DNA variation indicates low levels of genetic differentiation among cuttlefish (Sepia officinalis L.) populations in the English Channel and the Bay of Biscay. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. 1: 375-383.
Wright, S. 1978. Evolution and the genetics of populations.Variability within and among natural populations.Vol. 4. University of Chicago press, Chicago, IL, USA.
Zheng, X., Ikeda, M., Kong, L., Lin, X., Li, Q., Taniguchi, N. 2009. Genetic diversity and population structure of the golden cuttlefish, Sepia esculenta (Cephalopoda: Sepiidae) indicated by microsatellite DNA variations. Marine Ecology. 30: 448-454.

  • Receive Date 26 November 2013
  • Revise Date 17 October 2016
  • Accept Date 24 October 2016
  • Publish Date 23 August 2017