Journal of Marine Science and Technology

Journal of Marine Science and Technology

Molecular and phylogenetic analysis species Chiromantes boulengeri (Decapoda, Brachyura, Sesarmidae) from Arvand River

Document Type : Original Manuscript

Authors
N Nozarpour
M.B Nabavi
Ronagh M.T Ronagh
B Archangi
N Sakhai
Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr
10.22113/jmst.2016.34123
Abstract
In this study, the molecular characteristics of Chiromantes boulengeri in 2014 from intertidal waters of Arvand River in the vicinity of Minoo island were investigated. So, in October 2014 specimens from intertidal of two area of Minoo island were collected and preserved in 70% ethanol, carried out to the khoramshahr University of Marine Science and Technology laboratory and examined. To fulfill the purpose, To extract the DNA, phenol- chloroform method were used and mitochondrial gene 16S rRNA was sequenced and compare with other species from GenBank NCBI. The morphological and phylogeny sequencing results showed that all specimens belong to Chiromantes boulengeri in the study area. By polymorphism analysis, there are mutation within C. boulengeri from two area of Arvand River and it seems that this species for its survival has adapted with different ecological conditions in the study area.
Keywords
Chiromantes boulengeri
Arvand river
mt DNA
Phylogeny

Subjects

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  • Receive Date 21 May 2016
  • Revise Date 05 October 2016
  • Accept Date 15 October 2016
  • Publish Date 23 July 2018