Journal of Marine Science and Technology

Journal of Marine Science and Technology

Measurement of the lethal concentration of thyme essential oils in Rainbow trout (Onchorhynchus mykiss)

Document Type : Original Manuscript

Authors
1 Marine Chemistry, Faculty of Marine & Oceanic Sciences, University of Mazandaran, Babolsar
2 Toxicology, Faculty of Veterinary Medicine, University of Tehran,Tehran
3 Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran
Abstract
The importance of the use of medicinal plants against infectious and non-infectious diseases is completely obvious. The replacement of synthetic drugs due to their multiple properties in the prevention and treatment of many diseases, particularly in the aquaculture industry seems to be very important. This study was performed with the aim of assessing the acute toxicity (LC50-96) of thyme essential oil on rainbow trout.
To study the effect of the essential oils, 80 premature rainbow trout with an average weight of 10±0.5 in 9-liter tanks were exposed to different concentrations of essential oils of thyme and daily losses based on OECD protocol were calculated during 96 hours. All the physical and chemical factors such as water temperature, dissolved oxygen, pH, nitrite, nitrate, ammonium levels, electrical conductivity and water hardness were measured in different treatments. The acute toxicity of the essential oil of thyme was calculated using Probit analysis and a concentration of 4.4 ppm was determined.
Keywords

Subjects


Alishahi, M. 2004. Immune stimulant role in aquaculture. Journal of Veterinary Organization of Iran. 3: 33-38. (in persian).
Asadi, T., Zanguee, N., Mousavi, S.M. and Yavari, V. 2016. Effects of ginger extract on some hematological and serological parameters and growth performancein Barbus sharpeyi. Journal of Marine Science and technology. 15(1): 100-110. (in persian).
Dehghan, F., Vazirzadeh, A. and Karami, A. 2015. Effects of medicinal plants on growth rate, immune enhancement and diseases-resistance in cultured fish. The Third Iranian Conference of Ichthyology. May 6-7- 2015. Shiraz University. 194p.
Dügenci, S.K., Arda, N. and Candan, A. 2003. Some medicinal plants as immunostimulant for fish. Journal of Ethnopharmacology. 88(1): 99-106.
Finney, D.J. 1947. Probit Analysis. Cambridge University Press.
Firouzbakhsh, F., Nosrati, A. and Rahmati, N. 2015. Evaluation of Artemisia annua essential oils on growth performance in Astronotus ocellatus. The Third Iranian Conference of Ichthyology. 6-7 May 6-7- 2015. Shiraz University. 29p.
Jegede, T. 2007. Acute-toxicity of Sodium chloride (NaCl) on Oreochromis niloticus fingerlings. Journal of Fisheries International. 2(4): 292-294.
Mirsattari, G. 2002. Essentials of Toxicology. University of Tehran. Iran. 292p.
Mitchell, A.J. 1984. Parasites and Diseases of Striped Bass. University of Maryland, USA. 177p.
Moghaddam, S.B., Rohani, M.S., Sharifpour, I., Pajand, Z., Jalilpour, J. and Masoumzadeh, M. 2015. Determination of lethal concentration (LC96h) of hydroalcoholic extract of garlic (Allium sativum L.) and its effects on gill tissue of Persian sturgeon (Acipenser persicus) fingerlings. Iranian Journal of Medicinal and Aromatic Plants. 30(6): 915-922. (in persian).
Mousavi, S.M., Nasab, E.M., Yavari, V., Ghatarami, E.R. and Jalali, M.R. 2013. Determination of toxicity and mean lethal concentration (LC50) of Eugenol on Barbus sharpeyi. Iranian Journal of Medicinal and Aromatic Plants, 29(3): 551-560. (in persian).
Rohani, M.S., Haghighi, M. and Assaeian, H. 2011. The lethal concentration (LC50) of Zataria multiflora essential oil in fries of rainbow trout (Oncorhynchus mykiss). Iranian Scientific Fisheries Journal. 20(2): 89-96.
Salimian, F., Fattollahi, M., Nematollahi, A., Nikookhah, F. and Kharazian, N. 2017. Antifungal activity in vitro of aqueous and total flavnoids extracts of plant myrtus communis against two pathogenically important fungi, saprolegnia and fusarrium isolated from rainbow trout eggs. Journal of Marine Science and technology. 16(1): 16-28. (in persian).
Zarban, A., Malekaneh, M., Pourmohammad, H., Najari, M.T. and Abad, M. 2004. Evaluation of antioxidant properties of 28 Iranian medicinal plants. Journal of Birjand University of medical science. 11(1): 9-15. (in persian).

  • Receive Date 25 May 2016
  • Revise Date 05 July 2016
  • Accept Date 30 August 2016
  • Publish Date 22 May 2018