Inheritance of Microsatellite Markers in the hybrid of female Chalcalburnus chalcoides and male Vimba vimba persa Chalcalburnus chalcoides (Gueldenstaedt, 1772) ×Vimba vimba persa (Pallas, 1814)

Rouholahi, Shaghayegh; Zolgharnein, Hossein; Rezavani Gilkolaei, Sohrab; Salari Aliabadi, Mohammad Ali; Mohamadian, Samira; Taghavi, Mohammad Javad; Laluei, Faramarz; Shojaei, Hojat

Inheritance of Microsatellite Markers in the hybrid of female Chalcalburnus chalcoides and male Vimba vimba persa Chalcalburnus chalcoides (Gueldenstaedt, 1772) ×Vimba vimba persa (Pallas, 1814)

Document Type : Original Manuscript

Authors

Shaghayegh Rouholahi

Hossein Zolgharnein

Sohrab Rezavani Gilkolaei

Mohammad Ali Salari Aliabadi

Samira Mohamadian

Mohammad Javad Taghavi

Faramarz laluei

Hojat Shojaei

Abstract

The Mendelian Inheritance of produced hybrids from Chalcalburnus chalcoides and Vimba vimba persa was investigated using Microsatellite Markers. Genomic DNA from the produced larva and also small piece of dorsal and pectoral fin of their parents were extracted by Phenol-Chlorophorm method and PCR reaction was accomplished. 10 loci were amplified, in which 7 of them were amplified with reasonable polymorphism and 3 were monomorphism.
In order to study the segregation of the produced F1 hybrids from female Chalcalburnus chalcoides and male Vimba vimba persa, seven loci (LCO3,LCO1,Lid11,Rru2,Z1-2,Z7-8,Z9-10) were used.
According to the results it showed that parents and F1 larva‘s allelic distribution were almost segregated based on Mendelian inheritance in 7 loci. The present study showed that Microsatellite markers are useful marker in study of inheritance.

Keywords

Mendelian inheritance

Microsatellite markers

Chalcalburnus chalcoides

Vimba vimba persa