the genetic diversity assessment of Crassostrea sp. in the coasts of the Bandar_e Imam Khomeini using sequencing of 16S rRNA gene

Ahmadi, Zeynab; Zolgharein, Hossein; Archangi, Bita; Rajabzade, Ebrahim

doi:10.22113/jmst.2015.15666

the genetic diversity assessment of Crassostrea sp. in the coasts of the Bandar_e Imam Khomeini using sequencing of 16S rRNA gene

Document Type : Original Manuscript

Authors

zeynab ahmadi

hossein zolgharein

bita archangi

ebrahim rajabzade

10.22113/jmst.2015.15666

Abstract

16S rRNA is one of the components of the ribosome and widely used in phylogenetic studies of closely related species and especially related species Crassostrea. Nucleotide sequence polymorphism associated with 16S rRNA gene of mitochondrial genome of Persian Gulf Oyster (Crassostrea sp.), examined in 30 samples collected from Badar-e Emam Khomeini oysters. Through a primer PCR chain reaction was performed for all samples and product of PCR were Automated sequencing. Then the data obtained were analyzed by the programs and software. For the gene studied only there was 2 mutation and Nucleotide diversity (Pi) 0.00061 calculated. Among the 30 samples, 3 haplotypes were detected and average haplotype diversity (Hd) 0.195 were calculated. The haplotypes obtained in this study were first registered in the gene bank. Phylogeny trees drived from 16S rRNA gene sequencing showed no clear separation of the samples in the Bandar-e Imam Khomeini region. The results of this study showed that there is low sequence differentiation in the study population in Bandar-e Imam Khomeini.

Keywords

16S rRNA

Crassostrea

primer

PCR chain reaction

sequencing