Document Type : Original Manuscript
Authors
1 Department of Marine Animal, Faculty of Marine Science and Technology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
2 Department of Marine Biology, Faculty of Marine Science and Technology, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
3 South of Iran Aquaculture Fishery Research Center, Ahvaz, Iran.
Abstract
For this research, the required Acanthopagrus latus fish were selected by the expected catch method and finally the same size samples with an average weight of 150 g were selected. Then, five pieces of fish in 300 liter aquariums containing water with different salinities including Khor Musa with a mean salinity of 42.7 ± 0.34 ppt and Arvand with a mean salinity of 14.4 ± 0.29 ppt were exposed to 0.1 LC5096 obtained from the lethal toxicity of silver nanoparticles and silver nitrate. For each environment and control treatment, after two weeks of adaptation, they were placed with three replications. The sampling was performed at 1, 6, 12 and 24 hours from the caudal stalk vein with 2 cc syringes and 5 millimeter samples of liver at the end of the experiment. To separate the serum, blood samples without anticoagulant were centrifuged for 15 minutes at 3000 rpm. Finally, the activity of ALP, AST and ALT enzymes was measured and evaluated by an autoanalyzer device using diagnostic kits. Also, to prepare microscopic micrographs, paraffin sections of liver tissue were stained with hematoxylin-eosin. Results showed that two different forms of silver, including silver nitrate and silver nanoparticles, had different effects on the health and liver function of the Acanthopagrus latus which was evident in the treatment of silver nitrate. This action has been seen as a change in the activity of serum enzymes especially the increase in more times, necrosis, hyperemia, hemorrhage, biliary regurgitation and vacuolar degeneration especially at low salinities.
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