Fractionation comparison of Persian Gulf jellyfish nematocyst venom by two methods of chromatography

Parviz, M.; Nikpour, Yadollah; Taghavi Moghadam, A.; Ghanemi, K.

doi:10.22113/jmst.2017.14761

Fractionation comparison of Persian Gulf jellyfish nematocyst venom by two methods of chromatography

Document Type : Original Manuscript

Authors

M. Parviz ¹

yadollah nikpour ¹

A. Taghavi Moghadam ²

K. Ghanemi ¹

¹ Department of Marine Chemistry, College of Marine Science, Khorramshahr Marine Science and Technology University.

² Razi Vaccine and Serum Research Institute, Ahvaz, Iran.

10.22113/jmst.2017.14761

Abstract

In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, as the other species.
After extraction of nematocyst venom, the crude venom has been partially purification by sephadex G-200 gel filtration and DEAE anion exchange chromatography. Protein elution was monitored by uv detection at 280 nm. For determine the hemolytic fraction, every fraction were injected to 3 mice via the tail vein. Finally, all data from both chromatography methods were compared. The gel filtrations first pick and the first and second pick of anion exchange chromatography showed hemolytic activity.
Determination the appropriate method for purification of this venom can help to find a comprehensive method for another marine venom especially jellyfish venoms and eventually may be help to find specific antidote for jellyfish stings of these species.

Keywords

jellyfish

nematocyst

gel filtration chromatography

anion exchange chromatography

Crambionella Orsini

20.1001.1.20088965.1395.15.4.3.4

Subjects

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