Journal of Marine Science and Technology

Journal of Marine Science and Technology

Morphological and Molecular Identification of Brown Algae, Padina sp.(thivy, 1960) In Lengeh Port, Persian Gulf

Document Type : Original Manuscript

Authors
, A iTaherpour 1
bita archangi 2
S Ghaemmaghami 1
H Zolgharnein 1
K Ghanemi 3
1 Faculty of Marine Biology, Department of Marine Science and Oceanography, University of Marine Science and Technology
2 School of Marine Biology, Faculty of Marine Science and Oceanography, Khoramshahr University of Marine science and Technology
3 Faculty of Marine Chemistry, Department of Marine Science and Oceanography, University of Marine Science and Technology
10.22113/jmst.2016.11574
Abstract
Brown algae of Padina sp (Adanson, 1763) are found globally across tropical coastal areas, inter-tidal and sub-tidal regions. The aim of this research was to identify morphological and molecular characteristics of padina species distributed across Lengeh Port in Persian Gulf using morphological examinations and rbcL chloroplast gene sequencing. For this purpose, morphological features were undertaken using valid identification keys. For molecular analysis, total genomic DNA was extracted by CTAB method with slight modifications. Amplification of fragments was carried out using rbcL primers. Analysis of genome sequences was undertaken using genetic softwares of Chromas, BioEdit and MEGA6 and phylogenetic trees were constructed by two models of Neighbor Joining (NJ) and Maximum Likelihood (ML). Results indicated that two identical species of padina (P. boergesenii and P. australis) in this research shared common cluster with 99% probability and showed high genetic similarities among related compared padina species submitted in NCBI Genbank.
Keywords
P. australis
P. boergessenii
rbcL
Lengeh Port
Persian Gulf

Subjects

Gherinjak, BM., Ruhani Ghadikolaii K. 2011. Atlas of Marine Algae from Coasts of Persian Gulf and Oman Sea. Ministry of Agriculture. Iranian Fisheries Institute. 202 pp.
Abbas, A., Shameel, M. 2013. Morpho-anatomical Studies on the Genus Padina (Dictyotales, Phaeophycota) from the Coast of Karachi. Pakistan. Pakistan Academy of Science 50: 21-36.
Allender, B., Kraft, G. 1983. The marine algae of Lord Howe Island (NSW): The dictyotales and Cutleriales (Phaeophyta). Aust. Syst. Bot. 6: 73-130.
Amini, F., Riahi, H., Zolgharnain, H. 2013. Ribulose-1, 5-Bisphosphate Carboxylase/Oxygenase Gene Sequencing in Taxonomic Delineation of Padina Species in the Northern Coast of the Persian Gulf, (IRAN). J. of the Persian Gulf 4: 47-57.
De Clerck, O., Leliaert, F., Verbruggen, H., Lane, CE. De Paula, J.C., Payo, D.A., Coppejans, E. 2006. A revised classification of the Dictyoteae (Dictyotales, Phaeophyceae) based on rbcL and 26S ribosomal DNA sequence analyses1. J. of Phycol. 42: 1271-1288.
Doyle, J.J., Poyle, J.L., Ballenger, J.A., Dickson, E.E., Kajita, T., Ohashi, H. 1997. A phylogenetic of the chloroplast gene rbcL in the Leguminosae: taxonomic correlations and insights into the evolution of nodulation. American J. of Bot. 84: 541–554.
Hamdam, N., Samad, A.A., Hidyat, T., Salleh, F.M. 2013. Phylogenetic analysis of eight Malaysian pineapple cultivars using a chloroplast marker (rcbL gene). J. of Tech. 64: 29–33.
Hao, D.C., Mu, J., Xiao, P.G. 2010. Molecular evolution and positive Darwinian selection of the gymnosperm photosynthetic Rubisco enzyme. Bot. Stu. 51: 491-510.
Hoshina, R., Hasegawa, K., Tanaka, J., & Hara, Y. 2004. Molecular phylogeny of the Dictyotaceae (Phaeophyceae) with emphasis on their morphology and its taxonomic implication. Jap. J. of Phycol. 52(suppl): 189–94.
Lee, W.J., & Bae, K.S. 2002. Phylogenetic relationships among several genera of Dictyotaceae (Dictyotales, Phaeophyceae) based on 18S rRNA and partial rbcL gene sequences. Mar. Biol. J. 140:1107–15.
Liang, H. 1997. The Phylogenetic reconstruction of the grass family (Poaceae) using matK gene sequences. Virginia Polytechnic Institute and State University.
Ni-Ni-Win,Hayuda, T., Arai, S., Uchimura, M., Prathep, A., Draisma, S.G.A., Phang, S.M., Abott, I.A., Millar, A.J.K., & Kawai, H. 2011a. A taxonomic study of the genus Padina (dictyotales, phaeophyceae) including the description of four new species from Japan, Hawaii and the Andaman sea. J. of Phycol 47: 1193–1209.
Ni-Ni-Win, Hayuda, T., Draisma, SGA. Furnal, G., Meinesz, A., & Kawai, H. 2011. Padina ditristromatica sp.nov. and Padina pavonicoides sp.nov. (Dictyotales, Phaeophyceae), two new species from the Mediterranean Sea based on morphological and molecular markers. European J. of Phycol 46: 327–341.
Sheng-Guo, J., Ke-Ke, H., Jun, W., Sheng-Li, P. 2008. A molecular phylogenetic study of Huperziaceae based on chloroplast rbcL and psbA-trnH sequences. J. of Syst. Evol. 46: 213–219.
Silberfeld, T., Bittner, L., Fernández‐García, C., Cruaud, C., Rousseau, F., Reviers, B., Leliaert, F., Payri, C.E., Clerck, O. 2013. Species diversity, phylogeny and large scale biogeographic patterns of the genus Padina (Phaeophyceae, Dictyotales). J. of Phycol 49: 130-142.
Sohrabipour, J., Nejadsatari, T., Assadi, M., Rabei, R. 2004. The marine algae of the southern coast of Iran, Persian Gulf, Lengeh area. Iranian J. of Bot. 10: 83-93.
Trono, C., Jr. 1969. The marine benthic algae of the Caroline Islands. II. Phaeophyta and Rhodophyta. Micronesica. 5: 25–119.
Trono, J.R., Gaviro, C. 1997. Field guide and atlas of the seaweed recourses of Philippines. Bookmark. Inc. Makati City, Philippines 306.
Tuney, Đ., Sukatar, A. 2010. DNA extraction protocol from Brown Algae. Biol. Div and Cons 3: 51-55.
Wongsawad, P., Peerapornpisal, Y. 2014. Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers. Saudi J. of Biol. Sci.
Journal of Marine Science and Technology
Volume 15, Issue 2
Autumn 2016
Pages 89-96

  • Receive Date 05 April 2015
  • Revise Date 25 May 2015
  • Accept Date 02 November 2015
  • Publish Date 22 August 2016