Genetic characterization of Sillago sp.  from Hormozgan coastal waters using microsatellite markers

Shadi, Ahmad; Zolgharnein, Hosein; Salari-aliabadi, MohamadAli; Nabavi, Mohamad Bagher; Ronagh, Mohamad Taghi

doi:10.22113/jmst.2014.7812

Genetic characterization of Sillago sp. from Hormozgan coastal waters using microsatellite markers

Document Type : Original Manuscript

Authors

Ahmad Shadi ¹

Hosein Zolgharnein ¹

MohamadAli Salari-aliabadi ²

Mohamad Bagher Nabavi ²

Mohamad Taghi Ronagh ²

¹ Department of Marine Biology, Khormashahr University of marine Science and Technology

² Department of Marine Biology,Khoramshahr University of Marine Science and Technology

10.22113/jmst.2014.7812

Abstract

Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 loci with 8.4 mean allelic frequency. Observed and expected heterozygosity values was calculated (0.115<Ho<0.792) ,(0.598<He<0.902) . Genetic distance and identity according to Nei was estimated 0.520 and 0.595 respectively which shows genetic distance of genus level. Significant deviation from Hardy-Weinberg equilibrium (p<0.001) was observed at all loci at two stations. Analysis of Molecular Variance(AMOVA) of Genetic differentiation index(Fst) revealed mean significant (p=0.001) differentiation between two studied regions and 3.90 gen flow. Despite low distance of two studied regions (180km) there are probably two distinct populations of Sillago sp. which should be considered in management efforts.

Keywords

Silaginids

microsatellites

Population genetics

Hormozgan

Persian Gulf