Document Type : Original Manuscript
Authors
1
Seafood Processing Department, Fisheries & Environment Faculty, Gorgan University of Agricultural Sciences & Natural Resources, Box: 4913815739, Gorgan, Iran.
2
Seafood Processing Department, Fisheries & Environment Faculty, Gorgan University of Agricultural Sciences & Natural Resources, Box: 4913815739, Gorgan, Iran.
3
Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, P.O. Box: 578, Sari, Iran.
4
Seafood Processing Department, Fisheries & Environment Faculty, Gorgan University of Agricultural Sciences & Natural Resources, Box: 4913815739, Gorgan, Iran.
10.22113/jmst.2025.486357.2621
Abstract
This study aimed to identify in-paralog genes (genes derived from a common ancestral DNA sequence) in five strains, including 53789_strain, C052_strain, C057_strain, CCNP1411_strain, and MS1_strain, using bioinformatics approaches and genomic data from the genus Nostoc. Based on the OrthoMCL analysis, a total of 35,037 proteins were identified, among which 3,056 singleton proteins were detected, indicating the absence of duplication events for these proteins during the evolutionary process. In addition, more than 3,430 orthologous gene groups were identified across the five Nostoc strains, reflecting the divergence of a gene following a speciation event while retaining the gene's original function. 301 paralogous proteins were also identified among the five strains, signifying the persistence of these genes during the processes of evolution and speciation. The analysis of strain similarities based on protein homology revealed the highest similarity between the C057 and ATCC_53789 strains. Subsequently, the extraction and purification processes were carried out, followed by the evaluation of their antimicrobial and antioxidant effects. Overall, the documentation of shared genes revealed that some of these genes participate in critical biological pathways, including cellular processes, DNA metabolic processes, chromatin organization, responses to environmental stimuli, growth, and the cell cycle.
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